Get access

Anthocyanin-rich Fractions of Blackberry Extracts Reduce UV-induced Free Radicals and Oxidative Damage in Keratinocytes

Authors

  • Patience Murapa,

    1. University of Kentucky College of Medicine, Department of Pediatrics, The Markey Cancer Center and the Graduate Center for Toxicology, Department of Molecular and Biomedical Pharmacology, Lexington, KY, USA
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Jin Dai,

    1. Four Tigers LLC, 1501 Bull Lea Road, Suite 105, Lexington, KY 40511 and the Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, USA
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Michael Chung,

    1. University of Kentucky College of Medicine, Department of Pediatrics, The Markey Cancer Center and the Graduate Center for Toxicology, Department of Molecular and Biomedical Pharmacology, Lexington, KY, USA
    Search for more papers by this author
  • Russell J. Mumper,

    1. Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, CB # 7360, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Search for more papers by this author
  • John D'Orazio

    Corresponding author
    • University of Kentucky College of Medicine, Department of Pediatrics, The Markey Cancer Center and the Graduate Center for Toxicology, Department of Molecular and Biomedical Pharmacology, Lexington, KY, USA
    Search for more papers by this author

Dr John D'Orazio, Associate Professor of Pediatrics, University of Kentucky College of Medicine, Markey Cancer Center, Combs Research Building 204, 800 Rose Street, Lexington, KY 40536-0096, USA.

E-mail: jdorazio@uky.edu

Abstract

Hull blackberries were purified using solid phase extraction to obtain anthocyanin-rich methanol fractions. This method concentrated phenolics and anthocyanins, recovering 97% and 76% of the total yield in puree or powder extracts, respectively, which represented a 24–63 fold increase of the total antioxidant capacity when compared with either the water fraction or the original extract. The ability of these fractions to protect primary keratinocytes against UV-induced oxidative damage was assessed. Anthocyanin-rich methanol fractions derived from either blackberry powder or puree exhibited strong antioxidant properties, protecting against UV-induced ROS nearly as efficiently as N-acetyl cysteine. Furthermore, the fractions up-regulated the expression of catalase, MnSOD, Gpx1/2 and Gsta1 antioxidant enzymes. Thus, it is concluded that blackberry extracts may protect keratinocytes against UV-mediated oxidative damage. Copyright © 2011 John Wiley & Sons, Ltd.

Ancillary