An Aqueous Extract of Withania somnifera Root Inhibits Amyloid β Fibril Formation In Vitro

Authors

  • Suresh Kumar,

    1. Faculty of Science, Agriculture and Engineering, School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, UK
    2. University School of Biotechnology, GGS Indraprastha University, Dwarka, New Delhi, India
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  • Robin J. Harris,

    1. Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
    2. Institute of Zoology, University of Mainz, Mainz, Germany
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  • Christopher J. Seal,

    1. Faculty of Science, Agriculture and Engineering, School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, UK
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  • Edward J. Okello

    Corresponding author
    1. Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, UK
    • Faculty of Science, Agriculture and Engineering, School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, UK
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Dr Ed Okello, Medicinal Plant Research Group, School of Agriculture, Food and Rural Development, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK.

E-mail: e.j.okello@ncl.ac.uk

Abstract

The ability of an aqueous extract of W. somnifera L. Dunal (Family: Solanaceae) roots to inhibit fibril formation by the amyloid-β peptide in vitro was investigated. W. somnifera is used extensively in traditional Ayurvedic medicine as a nerve tonic with reputed memory enhancing properties. Inhibition of fibrillogenesis measured by transmission electron microscopy and ThT fluorescence assay showed that an aqueous extract of W. somnifera strongly inhibited Aβ fibril formation in a concentration-dependent manner, when compared with control samples. These results suggest that the aqueous extract of W. somnifera root has an ability to inhibit the formation of mature amyloid-β fibrils in vitro, which are known to lead to amyloid plaque formation in vivo. Copyright © 2011 John Wiley & Sons, Ltd.

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