Linalool from Rosewood (Aniba rosaeodora Ducke) Oil Inhibits Adenylate Cyclase in the Retina, Contributing to Understanding its Biological Activity

Authors

  • Lucia de Fatima S. Sampaio,

    Corresponding author
    • Laboratório de Bioquímica do Desenvolvimento do Sistema Nervoso, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil
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  • José Guilherme S. Maia,

    1. Faculdade de Engenharia Química, Instituto de Tecnologia, Universidade Federal do Pará, Belém, PA, Brazil
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  • Amanda M. de Parijós,

    1. Laboratório de Bioquímica do Desenvolvimento do Sistema Nervoso, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil
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  • Rita Z. de Souza,

    1. Laboratório de Pesquisa e Desenvolvimento de Produtos Naturais, Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brazil
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  • Lauro Euclides S. Barata

    1. Laboratório de Pesquisa e Desenvolvimento de Produtos Naturais, Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brazil
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Professor Lucia de Fatima S. Sampaio, Laboratório de Bioquímica do Desenvolvimento do Sistema Nervoso, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa 1, CEP 66075–110 Belém, PA, Brazil.

E-mail: lsampaio@ufpa.br

Abstract

Rosewood oil (RO) (Aniba rosaeodora Ducke) is rich in linalool, a monoterpene alcohol, which has well studied anxiolytic, sedative and anticonvulsant effects. The inhibition of the increases in cAMP protects against seizures in a diversity of models of epilepsy. In this paper, the principal aim was to investigate the effects of RO, (±)-linalool and (-)-linalool) on adenylate cyclase. They were tested in chick retinas and forskolin was used to stimulate the enzyme target. The phosphodiesterase inhibitor, 4-(3-butoxy-4-methoxybenzyl)-imidazolidin-2-one, and the non-selective adenosine receptor antagonist 3-isobutyl-methyl-xanthine (IBMX), were used to control the participation of phosphodiesterase and adenosine receptors in the resulting effects, respectively. The cAMP accumulation was measured by enzyme immune assay (EIA). Rosewood oil, (-)-linalool and (±)-linalool inhibited exclusively the cAMP accumulation stimulated by forskolin, even when adenosine receptors were blocked with IBMX. The IC50 values (in μ m concentration range) calculated from their concentration response–curves were not statistically different, however, the compounds presented a different relative efficacy. These results extend the range of subcellular mechanisms underlying the relaxant action of linalool on the central nervous system. Copyright © 2011 John Wiley & Sons, Ltd.

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