Urinary 1H-NMR Metabonomics Study on Intervention Effects of Soya Milk in Africans
Article first published online: 12 JUL 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 1, pages 127–135, January 2012
How to Cite
Ogegbo, O. L., Dissanyake, W., Crowder, J., Asekun, O., Familoni, O., Branford-White, C. J. and Annie Bligh, S. W. (2012), Urinary 1H-NMR Metabonomics Study on Intervention Effects of Soya Milk in Africans. Phytother. Res., 26: 127–135. doi: 10.1002/ptr.3547
- Issue published online: 6 JAN 2012
- Article first published online: 12 JUL 2011
- Manuscript Accepted: 7 MAY 2011
- Manuscript Received: 25 FEB 2011
- endogenous metabolites;
- statistical analysis
Metabonomics is an important tool in understanding the toxicological or therapeutic effects of interventions by analysing metabolic profiles and interpreting complex multi-dimensional spectroscopic/spectrometric data using multivariate data analysis. The objectives of this study were to evaluate the metabolic changes following a short-term 5 day soya milk intervention, and to investigate factors that influence soy-phytoestrogen metabolism focused on Africans based in either UK or Nigeria. 1H-NMR metabonomics was applied to analyse urine samples collected at four phases I–IV (pre, days 3 and 5, and post) of the soy-intervention from African volunteers (n = 40 in total). Individual proton NMR spectra were visually and statistically assessed using multivariate analyses (MVA): principal component analysis (PCA) and (orthogonal-) partial-least square–discriminant analysis ((O-) PLS-DA). In addition, 22 endogenous metabolites were quantified using a Chenomx NMR suite. The results showed the levels of analysed endogenous metabolites (creatinine adjusted) present ranged from 4 µ m to 12 m m with large inter-subject variances in acetate, acetone, lactate and trimethylamine. The MVA results showed high inter-individuality and sampling variances based on PCA score plots, and demonstrated soy metabolism to be significantly influenced by location and gender by both PLS-DA and O-PLS-DA. Copyright © 2011 John Wiley & Sons, Ltd.