Studies on Antidiarrheal and Antispasmodic Activities of Lepidium sativum Crude Extract in Rats
Article first published online: 18 OCT 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 1, pages 136–141, January 2012
How to Cite
Rehman, N.-u., Mehmood, M. H., Alkharfy, K. M. and Gilani, A.-H. (2012), Studies on Antidiarrheal and Antispasmodic Activities of Lepidium sativum Crude Extract in Rats. Phytother. Res., 26: 136–141. doi: 10.1002/ptr.3642
- Issue published online: 6 JAN 2012
- Article first published online: 18 OCT 2011
- Manuscript Accepted: 17 JUL 2011
- Manuscript Revised: 11 JUL 2011
- Manuscript Received: 18 APR 2011
- Lepidium sativum;
- Ca++ antagonist
This study was aimed to provide the pharmacological basis for the medicinal use of Lepidium sativum in diarrhea using in vivo and in vitro assays. The seed extract of Lepidium sativum (Ls.Cr) at 100 and 300 mg/kg inhibited castor oil-induced diarrhea in rats. In isolated rat ileum, Ls.Cr (0.01–5 mg/mL) reversed carbachol (CCh, 1 µ m) and K+ (80 m m)-induced contractions with higher potency against CCh, similar to dicyclomine. Preincubation of rat ileum with a lower concentration of Ls.Cr (0.03 mg/mL) caused a rightward parallel shift in the concentration–response curves (CRCs) of CCh without suppression of the maximum response, while at the next higher concentration (0.1 mg/mL), it produced a non-parallel rightward shift with suppression of the maximum response, similar to that of dicyclomine. Ls.Cr shifted the CRCs of Ca++ to the right with suppression of the maximum response, similar to verapamil. These data suggest that Lepidium sativum seed extract possesses antidiarrheal and spasmolytic activities mediated possibly through dual blockade of muscarinic receptors and Ca++ channels, though additional mechanism(s) cannot be ruled out and this study explains its medicinal use in diarrhea and abdominal cramps. Copyright © 2011 John Wiley & Sons, Ltd.