Hot Water Extract of Adzuki (Vigna angularis) Suppresses Antigen-Stimulated Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells and Passive Cutaneous Anaphylaxis Reaction in Mice
Article first published online: 15 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 7, pages 1003–1011, July 2012
How to Cite
Itoh, T., Hori, Y., Atsumi, T., Toriizuka, K., Nakamura, M., Maeyama, T., Ando, M., Tsukamasa, Y., Ida, Y. and Furuichi, Y. (2012), Hot Water Extract of Adzuki (Vigna angularis) Suppresses Antigen-Stimulated Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells and Passive Cutaneous Anaphylaxis Reaction in Mice. Phytother. Res., 26: 1003–1011. doi: 10.1002/ptr.3660
- Issue published online: 5 JUL 2012
- Article first published online: 15 DEC 2011
- Manuscript Accepted: 29 AUG 2011
- Manuscript Revised: 26 JUL 2011
- Manuscript Received: 13 DEC 2010
- adzuki (Vigna angularis);
- hot water extract of adzuki;
- passive cutaneous anaphylaxis reaction
The hot water extract of adzuki (HWEA), which is produced as a byproduct in the adzuki bean boiling process, has anti-tumor, antioxidative, and anti-diabetic activities. In this study, we fractionated HWEA to 4 fractions using stepwise gradient column chromatography with water and ethanol, and demonstrated the effects of each fraction on antigen (Ag)-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells. The 40% ethanol eluate extract (EtEx.40) showed the strongest inhibition level of these fractions. To reveal the inhibitory mechanisms underlying degranulation by EtEx.40, we investigated intracellular reactive oxygen species (ROS) production, intracellular free Ca2+ concentration ([Ca2+]i), and early intracellular signaling pathways. Treatment with EtEx.40 markedly inactivated Lyn following Ag stimulation, resulting in the suppressions of intracellular elevation of [Ca2+]i and production of ROS. To identify the active compound in EtEx.40, we isolated 7 flavonoids from EtEx.40 and calculated their inhibition levels on Ag-stimulated degranulation. These flavonoids inhibited degranulation by about 25–60%. We further examined the in vivo effects of HWEA or EtEx.40 using a passive cutaneous anaphylaxis (PCA) reaction. Both extracts strongly suppressed the PCA reaction. These findings suggest that HWEA and/or EtEx.40 are beneficial for alleviating type I allergic symptoms. Copyright © 2011 John Wiley & Sons, Ltd.