Ampelopsin Inhibits H2O2-induced Apoptosis by ERK and Akt Signaling Pathways and Up-regulation of Heme Oxygenase-1
Article first published online: 6 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 7, pages 988–994, July 2012
How to Cite
Kou, X., Shen, K., An, Y., Qi, S., Dai, W.-X. and Yin, Z. (2012), Ampelopsin Inhibits H2O2-induced Apoptosis by ERK and Akt Signaling Pathways and Up-regulation of Heme Oxygenase-1. Phytother. Res., 26: 988–994. doi: 10.1002/ptr.3671
- Issue published online: 5 JUL 2012
- Article first published online: 6 DEC 2011
- Manuscript Accepted: 1 SEP 2011
- Manuscript Revised: 29 AUG 2011
- Manuscript Received: 4 DEC 2010
- heme oxygenase-1;
Oxidative stress plays an important role in neurodegenerative disorders. Ampelopsin, a flavonoid abundant in Rattan tea (Ampelopsis grossedentata), is a potent antioxidant and its neuroprotective effect against H2O2-induced apoptosis in PC12 cells is investigated here for the first time. It was found that treatment of cells with ampelopsin for 1 h significantly reduced the loss of vitality, LDH release and apoptosis and inhibited the formation of reactive oxygen species (ROS). Ampelopsin was able to prevent the activation of p38 induced by H2O2. In addition, up-regulation of heme oxygenase-1 (HO-1) expression by ampelopsin was shown to be both dose- and time-dependent. Mechanically, HO-1 expression induced by ampelopsin was found to be due to activation of the ERK and Akt signaling pathways, because it was almost completely blocked by the specific inhibitors of ERK and Akt. These results suggest that ampelopsin increases cellular antioxidant defense through activation of the ERK and Akt signaling pathways, which induces HO-1 expression and thereby protects PC12 cells from H2O2-induced apoptosis. Copyright © 2011 John Wiley & Sons, Ltd.