Lipid-soluble Extracts from Salvia miltiorrhiza Inhibit Production of LPS-induced Inflammatory Mediators via NF-κB Modulation in RAW 264.7 Cells and Perform Antiinflammatory Effects In Vivo

Authors

  • Meng Li,

    1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, PR China
    2. Tianjin Medical University General Hospital, Tianjin, PR China
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    • These authors contributed equally to this work.

  • Lei Zhang,

    1. Center for Drug Evaluation of State Food and Drug Administration, Beijing, PR China
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    • These authors contributed equally to this work.

  • Run-Lan Cai,

    1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, PR China
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  • Yuan Gao,

    1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, PR China
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  • Yun Qi

    Corresponding author
    • Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, PR China
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Yun Qi, Research Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, 151 North Ma Lian Wa Road, Haidian District, Beijing, PR China.

E-mail: yunqichai@sohu.com

Abstract

Salvia miltiorrhiza, a traditional Chinese herbal medicine, is used to treat various inflammatory diseases. In the present study, the antiinflammatory effects of S. miltiorrhiza lipid-soluble extracts (SMLE) were demonstrated in vitro and in vivo, along with its underlying mechanism of action. SMLE significantly inhibited the production of NO, TNF-α, IL-1β and IL-6 in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. SMLE also inhibited the LPS-induced degradation of IκB-α in the cytoplasm and the translocation of p65 to the nucleus in RAW 264.7 cells. In addition, SMLE inhibited the production of intracellular reactive oxygen species (ROS) and the surface expression of CD14 induced by LPS. In animal models, intraperitoneal administration of SMLE increased the survival rate of endotoxemia and sepsis in mice. The topical administration of SMLE significantly inhibited ear edema induced by PMA. It was found that SMLE inhibits the LPS-induced gene and protein expression of iNOS, TNF-α, IL-1β and IL-6 in macrophages by blocking NF-κB activation, and these effects are mediated, at least in part, through the inhibition of intracellular ROS generation and the surface expression of CD14. The results suggest a possible therapeutic application of SMLE in inflammatory diseases and provide scientific evidence in support of the traditional Chinese medical practice of treatment with S. miltiorrhiza. Copyright © 2012 John Wiley & Sons, Ltd.

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