Effects of Alpinumisoflavone and Abyssinone V-4′-Methyl Ether Derived from Erythrina lysistemon (Fabaceae) on the Genital Tract of Ovariectomized Female Wistar Rat
Article first published online: 20 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 7, pages 1029–1036, July 2012
How to Cite
Mvondo, M.A., Njamen, D., Tanee Fomum, S. and Wandji, J. (2012), Effects of Alpinumisoflavone and Abyssinone V-4′-Methyl Ether Derived from Erythrina lysistemon (Fabaceae) on the Genital Tract of Ovariectomized Female Wistar Rat. Phytother. Res., 26: 1029–1036. doi: 10.1002/ptr.3685
- Issue published online: 5 JUL 2012
- Article first published online: 20 DEC 2011
- Manuscript Accepted: 18 SEP 2011
- Manuscript Received: 5 SEP 2011
- estrogen receptor ligands;
- vaginal stratification;
- uterine growth
Erythrina lysistemon is an African medicinal plant used as a palliative for problems in women. The crude extract of this plant was shown to exhibit estrogenic effects on the female rat reproductive tract and on cell cuture. Using classic chromatographic methods, two compounds have been isolated as major constituents of this extract: alpinumisoflavone (1) and abyssinone V-4′-methyl ether (2). To determine whether both compounds are actives principles accounting for E. lysistemon effects, we applied the classic 3-day uterotrophic assay. We also carried out a ligand binding assay to determine whether the observed effects are estrogen receptor (ER) mediated. This study showed that whereas compound 1 displayed a fourfold preference for ERα, compound 2 bound ERα and ERβ with a same affinity. The in vivo study showed that compound 1 increased the uterine wet weight by 182.23% (p < 0.01) and 71.79% (p < 0.05) at doses of 0.1 and 1 mg/kg BW/day respectively. The uterine epithelium thickened dose-dependently. Vaginal epithelial height also increased by 369.97 and 226.76% respectively (p < 0.01). Compound 2 acted only on the vagina and increased vaginal epithelial height by 244.56% (p < 0.01) at 1 mg/kg BW/day. These results suggest that compounds 1 and 2 are endowed with estrogenic properties accounting, at least in part, for E. lysistemon effects. Copyright © 2011 John Wiley & Sons, Ltd.