Genistein Mitigates Radiation-induced Testicular Injury
Article first published online: 9 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 8, pages 1119–1125, August 2012
How to Cite
Kim, J.-S., Heo, K., Yi, J.-M., Gong, E. J., Yang, K., Moon, C. and Kim, S.-H. (2012), Genistein Mitigates Radiation-induced Testicular Injury. Phytother. Res., 26: 1119–1125. doi: 10.1002/ptr.3689
- Issue published online: 22 JUL 2012
- Article first published online: 9 DEC 2011
- Manuscript Accepted: 22 SEP 2011
- Manuscript Revised: 8 SEP 2011
- Manuscript Received: 1 JUN 2011
- Nuclear R&D Program of the Ministry of Education, Science and Technology. Grant Numbers: 50496–2011, 50596–2011
The present study investigated the radioprotective effect of a multifunctional soy isoflavone, genistein, with the testicular system. Genistein was administered (200 mg/kg body weight) to male C3H/HeN mice by subcutaneous injection 24 h prior to pelvic irradiation (5 Gy). Histopathological parameters were evaluated 12 h and 21 days post-irradiation. Genistein protected the germ cells from radiation-induced apoptosis (p < 0.05 vs vehicle-treated irradiated mice at 12 h post-irradiation). Genistein significantly attenuated radiation-induced reduction in testis weight, seminiferous tubular diameter, seminiferous epithelial depth and sperm head count in the testes (p < 0.05 vs vehicle-treated irradiated mice at 21 days post-irradiation). Repopulation and stem cell survival indices of the seminiferous tubules were increased in the genistein-treated group compared with the vehicle-treated irradiation group at 21 days post-irradiation (p < 0.01). The irradiation-mediated decrease in the sperm count and sperm mobility in the epididymis was counteracted by genistein (p < 0.01), but no effect on the frequency of abnormal sperm was evident. Reactive oxygen species (ROS) were evaluated using DCFDA method and exposure to irradiation elevated ROS levels in the testis and genistein treatment resulted in a significant attenuation of radiation-induced ROS production. The results indicate that genistein protects from testicular dysfunction induced by gamma-irradiation by an antiapoptotic effect and recovery of spermatogenesis. Copyright © 2011 John Wiley & Sons, Ltd.