A New Isoflavone Glycitein 7-O-beta-d-Glucoside 4''-O-methylate, isolated from Cordyceps militaris grown on Germinated Soybeans Extract, Inhibits EGF-induced Mucus Hypersecretion in the Human Lung Mucoepidermoid Cells
Article first published online: 9 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 26, Issue 12, pages 1807–1812, December 2012
How to Cite
Kim, J.-H., Park, D. K., Lee, C. H. and Yoon, D.-Y. (2012), A New Isoflavone Glycitein 7-O-beta-d-Glucoside 4''-O-methylate, isolated from Cordyceps militaris grown on Germinated Soybeans Extract, Inhibits EGF-induced Mucus Hypersecretion in the Human Lung Mucoepidermoid Cells. Phytother. Res., 26: 1807–1812. doi: 10.1002/ptr.4655
- Issue published online: 11 DEC 2012
- Article first published online: 9 MAR 2012
- Manuscript Accepted: 1 FEB 2012
- Manuscript Revised: 26 JAN 2012
- Manuscript Received: 19 OCT 2011
A new isoflavone glycitein 7-O-beta-d-glucoside 4''-O-methylate (CGLM) has been isolated recently from Cordyceps militaris grown on germinated soybean extract and has antioxidant activity. In the present study, CGLM was investigated for its suppression of airway mucous hyper-secretion in epidermal growth factor (EGF)-treated human lung mucoepidermoid cells. NCI-H292 cells were treated with CGLM for 1 h, followed by EGF treatment for 24 h. The decrease in cyclooxygenase-2 (COX-2) production was correlated with reduced levels of protein and mRNA of inducible matrix metalloproteinase 9 (MMP-9) and also MUC5AC gene expression. CGLM directly inhibited down-regulated NF-κB activity, and significantly inhibited the phosphorylation of p38 and ERK1/2 (p42/p44) in NCI-H292 cells. These results suggest that CGLM protects NCI-H292 cells from EGF-induced damage by down-regulation of COX-2, MMP-9 and MUC5AC gene expression, mediated via blocking the NF-kappa-B and p38/ERK MAPK pathways. Copyright © 2012 John Wiley & Sons, Ltd.