Inhibitory Effect of Phenylbutanoid-rich Zingiber cassumunar Extracts on Nitric Oxide Production by Murine Macrophage-like RAW264.7 Cells
Article first published online: 3 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 26, Issue 12, pages 1789–1792, December 2012
How to Cite
Kaewchoothong, A., Tewtrakul, S. and Panichayupakaranant, P. (2012), Inhibitory Effect of Phenylbutanoid-rich Zingiber cassumunar Extracts on Nitric Oxide Production by Murine Macrophage-like RAW264.7 Cells. Phytother. Res., 26: 1789–1792. doi: 10.1002/ptr.4661
- Issue published online: 11 DEC 2012
- Article first published online: 3 MAR 2012
- Manuscript Accepted: 6 FEB 2012
- Manuscript Revised: 21 JUL 2011
- Manuscript Received: 2 OCT 2010
- Zingiber cassumunar;
- anti-inflammatory; standardization;
- herbal extract
Four phenylbutanoids, (E)-4-(3,4-dimethoxyphenyl)but-3-en-l-ol (I), (E)-4-(3,4-dimethoxyphenyl)but-3-en-l-yl acetate (II), (E)-1-(3,4-dimethoxyphenyl)butadiene (III) and (E)-3-(3,4-dimethoxyphenyl)-4-[(E)-3,4-dimethoxystyryl]cyclohex-1-ene (IV), isolated from Zingiber cassumunar, were used as standard markers for quantitative determination and preparation of phenylbutanoid-enriched Z. cassumunar extracts (PZEs). A reversed-phase HPLC method was established for the simultaneous determination of the phenylbutanoids in Z. cassumunar extracts. Systematic extraction studies to maximize phenylbutanoid content revealed that hexane was the most appropriate solvent for extraction. A one-step purification of the hexane crude extract of Z. cassumunar, using silica gel vacuum chromatography, provided the PZEs. The content of phenylbutanoids in the PZEs was up to 48.3% w/w dry weight. The anti-inflammatory activity of PZEs via inhibition of nitric oxide production by murine macrophage-like RAW264.7 cells was stronger than those of the four individual phenylbutanoids, the crude hexane extract and the essential oil of Z. cassumunar. Copyright © 2012 John Wiley & Sons, Ltd.