Inhibitory Activities of Compounds from the Twigs of Garcinia hombroniana Pierre on Human Low-density Lipoprotein (LDL) Oxidation and Platelet Aggregation
Article first published online: 15 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 26, Issue 12, pages 1845–1850, December 2012
How to Cite
Saputri, F. C. and Jantan, I. (2012), Inhibitory Activities of Compounds from the Twigs of Garcinia hombroniana Pierre on Human Low-density Lipoprotein (LDL) Oxidation and Platelet Aggregation. Phytother. Res., 26: 1845–1850. doi: 10.1002/ptr.4667
- Issue published online: 11 DEC 2012
- Article first published online: 15 MAR 2012
- Manuscript Accepted: 17 FEB 2012
- Manuscript Revised: 16 FEB 2012
- Manuscript Received: 24 DEC 2011
- Garcinia hombroniana Pierre (Guttiferae);
- LDL antioxidation;
- antiplatelet aggregation
The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′-tetrahydroxy-4-methoxybenzophenone, 1,7-dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin-5-en-3β-ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper-mediated LDL oxidation and arachidonic acid (AA)-, adenosine diphosphate (ADP)-, collagen-induced platelet aggregation in vitro. Among the compounds tested, 3,5,3′,5′-tetrahydroxy-4-methoxybenzophenone and 1,7-dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half-maximal inhibitory concentration (IC50) values of 6.6 and 1.7 µm, respectively. 3,5,3′,5′-Tetrahydroxy-4-methoxybenzophenone exhibited strong activity on AA-, ADP- and collagen-induced platelet aggregation with IC50 values of 53.6, 125.7 and 178.6 µm, respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP-induced aggregation with IC50 value of 5.7 µm. Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP-induced platelet aggregation. Copyright © 2012 John Wiley & Sons, Ltd.