Protective Effects of Fermented Citrus Unshiu Peel Extract against Ultraviolet-A-induced Photoageing in Human Dermal Fibrobolasts
Article first published online: 15 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 26, Issue 12, pages 1851–1856, December 2012
How to Cite
Bae, J. T., Ko, H. J., Kim, G. B., Pyo, H. B. and Lee, G. S. (2012), Protective Effects of Fermented Citrus Unshiu Peel Extract against Ultraviolet-A-induced Photoageing in Human Dermal Fibrobolasts. Phytother. Res., 26: 1851–1856. doi: 10.1002/ptr.4670
- Issue published online: 11 DEC 2012
- Article first published online: 15 MAR 2012
- Manuscript Accepted: 15 FEB 2012
- Manuscript Revised: 2 FEB 2012
- Manuscript Received: 22 SEP 2011
- Citrus unshiu peel;
- Schizophyllum commune;
- matrix metalloproteinase-1;
- collagen biosynthesis
The aqueous extracts of Citrus unshiu peel containing flavonoid glycosides was used as co-substrate with Schizophyllum commune mycelia producing β-glucosidase and its biological activities were studied. β-glucosidase-produced S. commune mycelia converted the glycosides (narirutin and hesperidin) into aglycones (naringenin and hesperetin). The photoprotective potential of fermented C. unshiu peel extract with S. commune (S-CPE) was tested in human dermal fibroblasts (HDFs) exposed to UVA. It was revealed that S-CPE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with S-CPE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. The UVA irradiation raised the proportion of senescence-associated β-galactosidase (SA-β-gal) positive cells in comparison with the normal control group. The treatment of UVA-irradiated HDFs with S-CPE was shown to decrease the level of SA-β-gal (by approximately 45% at an S-CPE concentration 0.1%, w/v) compared with the UVA-irradiated HDFs. It was found that S-CPE containing hesperetin has notable collagen biosynthetic activity for fibroblasts, indicating that S-CPE can be promising cosmetic ingredients. Copyright © 2012 John Wiley & Sons, Ltd.