Antithrombin Effect of Polyphenol-Rich Extracts from Black Chokeberry and Grape Seeds
Article first published online: 4 APR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 1, pages 71–76, January 2013
How to Cite
Bijak, M., Saluk, J., Ponczek, M. B. and Nowak, P. (2013), Antithrombin Effect of Polyphenol-Rich Extracts from Black Chokeberry and Grape Seeds. Phytother. Res., 27: 71–76. doi: 10.1002/ptr.4682
- Issue published online: 7 JAN 2013
- Article first published online: 4 APR 2012
- Manuscript Accepted: 2 MAR 2012
- Manuscript Revised: 22 FEB 2012
- Manuscript Received: 2 JAN 2012
- thrombin inhibition;
- grape seeds;
Thrombin is a serine protease that cleaves the peptide bonds in proteins located on the carboxyl side of arginine. Thrombin plays a central role in thromboembolic diseases, which are the major cause of mortality. The aim of the study was to estimate the effects of plant extracts on proteolytic properties of thrombin. Thrombin was incubated with polyphenol-rich extracts from berries of Aronia melanocarpa or seeds of Vitis vinifera (0.5, 5, 50 µg/mL) and with polyphenols ((+)-catechin, (−)-epicatechin, gallic acid, chlorogenic acid, procyanidin B1, cyanidin, cyanidin 3-glucoside, quercetin). The in vitro experiments showed that both extracts in all used concentrations inhibited proteolytic activity of thrombin observed as inhibition of thrombin-induced fibrinogen polymerization, stabilized fibrin formation, and platelet aggregation. Moreover, thrombin amidolytic activity was inhibited by polyphenols belonging to the flavonoid class. Results presented in this study indicate that polyphenol-rich extracts from berries of A. melanocarpa and seeds of V. vinifera may become promising dietary supplements in the prevention of thrombotic states. Copyright © 2012 John Wiley & Sons, Ltd.