These authors contributed equally to this work.
Ferulate Protects the Epithelial Barrier by Maintaining Tight Junction Protein Expression and Preventing Apoptosis in Tert-Butyl Hydroperoxide-Induced Caco-2 Cells
Version of Record online: 20 MAY 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 3, pages 362–367, March 2013
How to Cite
Kim, H. J., Lee, E. K., Park, M. H., Ha, Y. M., Jung, K. J., Kim, M.-S., Kim, M. K., Yu, B. P. and Chung, H. Y. (2013), Ferulate Protects the Epithelial Barrier by Maintaining Tight Junction Protein Expression and Preventing Apoptosis in Tert-Butyl Hydroperoxide-Induced Caco-2 Cells. Phytother. Res., 27: 362–367. doi: 10.1002/ptr.4717
- Issue online: 6 MAR 2013
- Version of Record online: 20 MAY 2012
- Manuscript Accepted: 10 APR 2012
- Manuscript Revised: 3 APR 2012
- Manuscript Received: 25 DEC 2011
- epithelial integrity;
- oxidative stress;
- tight junction
Epithelial barrier function is determined by both transcellular and paracellular permeability, the latter of which is mainly influenced by tight junctions (TJs) and apoptotic leaks within the epithelium. We investigated the protective effects of ferulate on epithelial barrier integrity by examining permeability, TJ protein expression, and apoptosis in Caco-2 cells treated with tert-butyl hydroperoxide (t-BHP), a strong reactive species inducer. Caco-2 cells pretreated with ferulate (5 or 15 μM) were exposed to t-BHP (100 μM), and ferulate suppressed the t-BHP-mediated increases in reactive species and epithelial permeability in Caco-2 cells. Moreover, ferulate inhibited epithelial cell leakage induced by t-BHP, which was accompanied by decreased expression of the TJ proteins zonula occludens-1 and occludin. In addition, pretreatment with ferulate markedly protected cells against t-BHP-induced apoptosis, as evidenced by decreased nuclear condensation, cytochrome c release, and caspase-3 cleavage and an increased Bax/Bcl-2 ratio. These results suggest that ferulate protects the epithelial barrier of Caco-2 cells against oxidative stress, which results in increased epithelial permeability, decreased TJ protein expression, and increased apoptosis. The most significant finding of our study is the demonstration of protective, ferulate-mediated antioxidant effects on barrier integrity, with a particular focus on intracellular molecular mechanisms. Copyright © 2012 John Wiley & Sons, Ltd.