Get access

The Effects of an Antiosteoporosis Herbal Formula Containing Epimedii Herba, Ligustri Lucidi Fructus and Psoraleae Fructus on Density and Structure of Rat Long Bones Under Tail-Suspension, and its Mechanisms of Action

Authors

  • Wing-Sum Siu,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    3. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Hing-Lok Wong,

    1. Jockey Club Centre for Osteoporosis Care and Control, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Ching-Po Lau,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Wai-Ting Shum,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Chun-Wai Wong,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Si Gao,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Kwok-Pui Fung,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    3. School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Clara Bik-San Lau,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Leung-Kim Hung,

    1. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Chun-Hay Ko,

    Corresponding author
    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    • State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author
  • Ping-Chung Leung

    Corresponding author
    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    3. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    • Jockey Club Centre for Osteoporosis Care and Control, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Search for more papers by this author

Correspondence to: Chun-Hay Ko, E302a, Institute of Chinese Medicine, Science Centre East, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China. Ping-Chung Leung, Centre for Clinical Trials on Chinese Medicine, 5/F, School of Public Health Building, Prince of Wales Hospital, Shatin, NT, Hong Kong.

E-mails: gohey@cuhk.edu.hk; pingcleung@cuhk.edu.hk

Abstract

An innovative anti-osteoporosis herbal formula containing Epimedii Herba, Ligustri Lucidi Fructus and Psoraleae Fructus (ELP) has been previously shown its bone protecting effects in ovariectomized osteoporotic rats and also in post-menopausal osteopenic women. This study aimed to investigate the efficacy of ELP against bone loss during physical inactivity or weightlessness. A hindlimb unloading tail-suspended rat model was used for studying the effects of ELP on bone mineral density (BMD) and bone micro-architecture. For in vitro mechanistic studies, rat mesenchymal stem cells (MSCs) and mouse macrophage cells (RAW264.7) were used for studying the effects of ELP on osteogenic/adipogenic differentiations and osteoclastogenesis, respectively. Our data illustrated that ELP had a significant preventive effect against bone loss induced by tail-suspension (TS) at day 28 (p < 0.01) as indicated in the reduction in BMD loss and the preservation of bone micro-architecture. ELP could significantly promote the osteogenesis and suppress the adipogenesis (p < 0.05) in MSCs. Besides, significant inhibition of osteoclast formation (p < 0.01) was found in ELP-treated RAW264.7 cells upon receptor activator of nuclear factor kappa-B ligand induction. Our study presents the first scientific evidence that ELP had a significant preventive effect against bone loss induced by TS through the actions of enhancing osteogenesis, suppressing adipogenesis and osteoclastogenesis. Copyright © 2012 John Wiley & Sons, Ltd.

Get access to the full text of this article

Ancillary