The Effect of the Ginkgo biloba Extract in the Expression of Bax, Bcl-2 and Bone Mineral Content of Wistar Rats with Glucocorticoid-Induced Osteoporosis
Version of Record online: 31 MAY 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 4, pages 515–520, April 2013
How to Cite
Lucinda, L. M. F., Aarestrup, B. J. V., Peters, V. M., de Paula Reis, J. E., de Oliveira, R. S. M. F. and de Oliveira Guerra, M. (2013), The Effect of the Ginkgo biloba Extract in the Expression of Bax, Bcl-2 and Bone Mineral Content of Wistar Rats with Glucocorticoid-Induced Osteoporosis. Phytother. Res., 27: 515–520. doi: 10.1002/ptr.4747
- Issue online: 12 APR 2013
- Version of Record online: 31 MAY 2012
- Manuscript Accepted: 30 APR 2012
- Manuscript Received: 19 APR 2012
- Ginkgo biloba;
- Bax expression;
- Bcl-2 expression;
- X-ray density
Objective: Evaluate the effects of the extract of Ginkgo biloba (EGb) in the glucocorticoid-induced-osteoporosis through the Bax and Bcl-2 expressions by osteoblast cells, the x-ray and bone density of the tibia. Method: Rats were divided into five groups: osteoporosis; EGb1 (28 mg/kg); EGb2 (56 mg/kg); alendronate (0.2 mg/animal) and control. The treatments were conducted for 20 (n = 30) and 30 days (n = 30). The Bax and Bcl-2 expressions were evaluated in osteoblasts of the mandibular alveolar bone. The tibias were radiographed to evaluate the X-ray and bone density. The control group was compared with the osteoporosis' (Student's t-test/Mann-Whitney). The other groups were analyzed by analysis of variance test followed by Dunnett/Dunnett T3 (p < 0.05). Results: When compared the osteoporosis to the control group (p <0.05): Bax and x-ray density increased; Bcl-2 and the bone density reduced. When compared with the osteoporosis group (p < 0.05), alendronate (30 days), EGb1 and EGb2 (20/30 days) increased the Bcl-2 expression; EGb2 and alendronate (20 days) EGb1 and EGb2 (30 days) reduced the Bax expression; and EGb1 and EGb2 (20/30 days) reduced the X-ray density. Conclusions: The EGb improved the Bcl-2 and reduced the Bax expression by osteoblasts in the mandibular alveolar bone and recovered the mineral content in the tibia of rats with glucocorticoid-induced-osteoporosis. Copyright © 2012 John Wiley & Sons, Ltd.