In Vitro and In Vivo Inhibitory Activities of Four Indian Medicinal Plant Extracts and their Major Components on Rat Aldose Reductase and Generation of Advanced Glycation Endproducts
Article first published online: 24 JUL 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 5, pages 753–760, May 2013
How to Cite
Rao, A. R., Veeresham, C. and Asres, K. (2013), In Vitro and In Vivo Inhibitory Activities of Four Indian Medicinal Plant Extracts and their Major Components on Rat Aldose Reductase and Generation of Advanced Glycation Endproducts. Phytother. Res., 27: 753–760. doi: 10.1002/ptr.4786
- Issue published online: 17 APR 2013
- Article first published online: 24 JUL 2012
- Manuscript Accepted: 27 JUN 2012
- Manuscript Received: 23 JUN 2012
- standardized plant extracts;
- enzyme inhibition;
- aldose reductase;
- advanced glycation endproducts;
- galactitol accumulation
The polyol enzyme aldose reductase (AR) and advanced glycation endproducts (AGEs) play an important role in diabetic complications such as cataracts. The purpose of this study was to investigate four standardized plant extracts used for the treatment of diabetes and related diseases, and their principal components for AR inhibitory activity and to find out their influence in diabetic complications. Thus, Boswellia serrata Triana & Planch. (Burseraceae), Lagerstroemia speciosa (L.) Pers. (Lythraceae), Ocimum gratissimum (L.) (Lamiaceae) and Syzygium cumin (L.) Skeels. (Myrthaceae) and their respective major constituents, boswellic acid, corosolic acid, ursolic acid and ellagic acid, were studied for their inhibitory activity against rat lens AR, rat kidney AR, human recombinant AR and generation of AGEs. In addition, in vivo inhibition of lens galactitol accumulation by the major constituents of the plants in galactose-fed rat has been studied. The results revealed that all the tested extracts and their active ingredients possess significant AR inhibitory actions in both in vitro and in vivo assays with urosolic acid showing the most potent effect. Furthermore, the study indicates the potential of the studied plants and their major constituents as possible protective agents against long-term diabetic complications. Copyright © 2012 John Wiley & Sons, Ltd.