Phytochemicals Isolated from Leaves of Chromolaena odorata: Impact on Viability and Clonogenicity of Cancer Cell Lines
Version of Record online: 16 AUG 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 27, Issue 6, pages 835–840, June 2013
How to Cite
Kouamé, P. B.-K., Jacques, C., Bedi, G., Silvestre, V., Loquet, D., Barillé-Nion, S., Robins, R. J. and Tea, I. (2013), Phytochemicals Isolated from Leaves of Chromolaena odorata: Impact on Viability and Clonogenicity of Cancer Cell Lines. Phytother. Res., 27: 835–840. doi: 10.1002/ptr.4787
- Issue online: 7 JUN 2013
- Version of Record online: 16 AUG 2012
- Manuscript Accepted: 11 JUL 2012
- Manuscript Revised: 26 JUN 2012
- Manuscript Received: 26 MAR 2012
- anticancer agent;
- Chromolaena odorata;
The leaves of Chromolaena odorata (Asteraceae) are exploited extensively in West and Central African ethnopharmacy for the treatment of a wide range of conditions, despite this being a non-native species established in the last 50 years. With the objective of seeking bioactive principles, the nonvolatile compounds, an ethanolic (80% v/v) extract was made and fractionated. From the hexane-soluble fraction, three compounds were isolated. Two of these, 5-hydroxy-7,4′-dimethoxyflavanone and 2′-hydroxy-4,4′,5′,6′-tetramethoxychalcone, have previously been identified in C. odorata leaves. The third was fully characterised spectroscopically and found to be 1,6-dimethyl-4-(1-methylethyl)naphthalene (cadalene), not previously isolated from the Asteraceae. All three compounds were tested for their cytotoxicity and anticancer properties. 2′-Hydroxy-4,4′,5′,6′-tetramethoxychalcone was found to be both cytotoxic and anticlonogenic at 20 µm in cell lines Cal51, MCF7 and MDAMB-468, and to act synergistically with the Bcl2 inhibitor ABT737 to enhance apoptosis in Cal51 breast cancer cells. Copyright © 2012 John Wiley & Sons, Ltd.