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Sanguisorbae Radix Protects Against 6-Hydroxydopamine-induced Neurotoxicity by Regulating NADPH Oxidase and NF-E2-related Factor-2/Heme Oxygenase-1 Expressions

Authors

  • Hyein Oh,

    1. Department of Life and Nanopharmaceutical Science, College of Pharmacy and Kyung Hee East–West Pharmaceutical Research Institute, Kyung Hee University, Seoul, Republic of Korea
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    • These authors contributed equally to this study.
  • Jinyoung Hur,

    1. Department of Brain Korea 21 Project Center, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
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    • These authors contributed equally to this study.
  • Gunhyuk Park,

    1. Department of Life and Nanopharmaceutical Science, College of Pharmacy and Kyung Hee East–West Pharmaceutical Research Institute, Kyung Hee University, Seoul, Republic of Korea
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    • These authors contributed equally to this study.
  • Hyo Geun Kim,

    1. Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
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  • Young Ock Kim,

    1. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong-gun, Chungcheongbuk-do, Republic of Korea
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  • Myung Sook Oh

    Corresponding author
    1. Department of Life and Nanopharmaceutical Science, College of Pharmacy and Kyung Hee East–West Pharmaceutical Research Institute, Kyung Hee University, Seoul, Republic of Korea
    • Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
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Correspondence to: Myung Sook Oh, Department of Oriental Pharmaceutical Science, College of Pharmacy and Kyung Hee East–west Pharmaceutical Research Institute, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 130–701, Korea.

E-mail: msohok@khu.ac.kr

Abstract

6-Hydroxydopamine (6-OHDA) produces neuronal cell damage by generating reactive oxygen species (ROS). The major mechanisms of protection against ROS-induced stress are inhibiting expression of ROS generating genes such as NADPH oxidase (NOX) and increasing expression of endogenous antioxidant genes such as heme oxygenase-1 (HO-1). This study investigated whether a standardized Sanguisorbae Radix extract (SRE), a medical herb commonly used in Asian traditional medicine, has a protective effect on 6-OHDA-induced cell toxicity by regulating ROS in SH-SY5Y cells. SRE at 10 and 50 µg/mL significantly reduced 6-OHDA-induced cell damage dose dependently in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and by Hoechst 33342 staining. SRE increased the B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X ratio and decreased cytochrome C release and caspase-3 activity. SRE also abolished 6-OHDA-induced ROS by inhibiting NOX expression and by inducing HO-1 expression via NF-E2-related factor-2 activation. Taken together, these results demonstrate that SRE has protective effects against 6-OHDA-induced cell death by regulating ROS in SH-SY5Y cells. Copyright © 2012 John Wiley & Sons, Ltd.

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