L-theanine Administration Results in Neuroprotection and Prevents Glutamate Receptor Agonist-Mediated Injury in the Rat Model of Cerebral Ischemia-Reperfusion
Article first published online: 24 OCT 2012
Copyright © 2012 John Wiley & Sons, Ltd.
How to Cite
Zukhurova, M., Prosvirnina, M., Daineko, A., Simanenkova, A., Petrishchev, N., Sonin, D., Galagudza, M., Shamtsyan, M., Juneja, L. R. and Vlasov, T. (2012), L-theanine Administration Results in Neuroprotection and Prevents Glutamate Receptor Agonist-Mediated Injury in the Rat Model of Cerebral Ischemia-Reperfusion. Phytother. Res.. doi: 10.1002/ptr.4868
- Article first published online: 24 OCT 2012
- Manuscript Accepted: 2 OCT 2012
- Manuscript Revised: 10 SEP 2012
- Manuscript Received: 5 JUL 2012
- brain ischemia;
While the neuroprotective effect of green tea (Camellia sinensis) might be explained by the presence of amino acid L-theanine in the tea leaves, it is not known whether postischemic administration of L-theanine could also provide neuroprotection. In the present study, we investigated the neuroprotective effect of L-theanine (1 and 4 mg/kg) administered at 3, 12, and 24 h after reperfusion in the rat model of stroke. We also studied the effect of L-theanine on brain injury caused by exogenous administration of N-methyl-D-aspartate and α-amino-3-hydroxy-5-methyl-isoxazole-4-propionate/kainate receptor agonists during reperfusion. Rats were subjected to 30-min middle cerebral artery occlusion followed by 48-h reperfusion. Neurological deficit and infarct size were determined at the end of reperfusion. At 3 and 12 h, but not at 24 h of reperfusion, L-theanine substantially reduced the size of brain infarct. Neurological status was improved when L-theanine was administered 3, 12, and 24 h after reperfusion. Repeated intrastriatal injections of L-theanine at a total dose of 800 µg/kg during reperfusion prevented brain injury caused by glutamate receptor agonists. In conclusion, L-theanine at reperfusion exerts neuroprotective effect in the in vivo rat model of stroke. Local treatment with L-theanine at reperfusion prevents glutamate receptor agonist-mediated brain injury. Copyright © 2012 John Wiley & Sons, Ltd.