Zhen Tian, Shui-bing Liu, and Yu-cai Wang contributed equally to this work.
Neuroprotective Effects of Formononetin Against NMDA-Induced Apoptosis in Cortical Neurons
Article first published online: 30 JAN 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 27, Issue 12, pages 1770–1775, December 2013
How to Cite
Tian, Z., Liu, S.-b., Wang, Y.-c., Li, X.-q., Zheng, L.-h. and Zhao, M.-g. (2013), Neuroprotective Effects of Formononetin Against NMDA-Induced Apoptosis in Cortical Neurons. Phytother. Res., 27: 1770–1775. doi: 10.1002/ptr.4928
- Issue published online: 13 DEC 2013
- Article first published online: 30 JAN 2013
- Manuscript Accepted: 19 DEC 2012
- Manuscript Revised: 21 NOV 2012
- Manuscript Received: 16 AUG 2011
- National Natural Science Foundation of China. Grant Numbers: 31070923, 31271126, 2011KTCL03-12, 2011ZXJ09106-01C
Formononetin (FMNT) is an isoflavone found in many herbs including Trifolium pratense L., Spatholobus suberectus Dunn., and Astragalus mongholicus Bunge. The purpose of this study is to investigate pharmacological properties of FMNT on neurotoxicity induced by N-methyl-D-asparate (NMDA) in primary-cultured cortical neurons. The cell viability was significantly decreased after exposure to NMDA (200 μM) for 40 min. Pretreatment of FMNT (10 μM) for 12 h significantly attenuated the cell loss induced by NMDA exposure. Flow cytometry analysis revealed that treatment of FMNT attenuated the number of apoptotic cells, especially the early phase apoptotic cells, induced by NMDA exposure. Western blot analysis showed that FMNT regulated the expression of apoptosis-related proteins by increasing the levels of Bcl-2 and pro-caspase-3 and decreasing the levels of Bax and caspase-3. These findings demonstrate that FMNT is capable of protecting neurons from NMDA-evoked excitotoxic injury and has a potential perspective to the clinical treatment for neurodegenerative disorders in central nervous system. Copyright © 2013 John Wiley & Sons, Ltd.