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Antiplatelet Activity of a Novel Formula Composed of Malic Acid, Succinic Acid and Citric Acid from Cornus officinalis Fruit

Authors

  • Qi-Chun Zhang,

    1. Department of clinic pharmacology, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P.R. China
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  • Yue Zhao,

    1. Department of clinic pharmacology, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P.R. China
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  • Hui-Min Bian

    Corresponding author
    1. Department of clinic pharmacology, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P.R. China
    • Correspondence to: Hui-Min Bian, Department of Clinical Pharmacology, School of Pharmacy, Nanjing University of Chinese Medicine, 282 Han-zhong Road, Nanjing, 210029, P. R. China.

      E-mail: hmbian@sina.com

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Abstract

The present study investigated the antiplatelet activity of a novel formula composed by malic acid, succinic acid and citric acid with a ratio of 3:2:2. The IC50 and inhibition of platelet aggregation induced by various agonists as well as platelet adhesion were evaluated in vitro. Of note, the IC50 for the formula inhibiting adenosine diphosphate (ADP)-induced platelet aggregation was 0.185 mg/mL. Meanwhile, the formula showed more potent inhibitory effect on platelet aggregation induced by ADP and thrombin than the single component at same concentration (0.37 mg/mL). Moreover, the formula could prevent platelet adhesion significantly without influence on platelet viability. Copyright © 2013 John Wiley & Sons, Ltd.

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