Neuroprotective Effects of Puerarin on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's Disease Model in Mice
Article first published online: 20 MAR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 28, Issue 2, pages 179–186, February 2014
How to Cite
Zhu, G., Wang, X., Wu, S., Li, X. and Li, Q. (2014), Neuroprotective Effects of Puerarin on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's Disease Model in Mice. Phytother. Res., 28: 179–186. doi: 10.1002/ptr.4975
- Issue published online: 4 FEB 2014
- Article first published online: 20 MAR 2013
- Manuscript Accepted: 20 FEB 2013
- Manuscript Revised: 18 FEB 2013
- Manuscript Received: 5 SEP 2012
- Parkinson's disease;
- glial cell line-derived neurotrophic factor;
- reactive oxygen species;
- Lamp 2A
Puerarin, an active component of Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep is well-known for its anti-oxidative and neuroprotective activities. Although anti-Parkinson's disease activity of puerarin was reported in both of in vivo and in vitro model, detailed mechanisms are not clarified. In this study, we addressed that puerarin attenuated 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced behavioral deficits, dopaminergic neuronal degeneration and dopamine depletion. Puerarin administration enhanced glutathione (GSH) activity, glial cell line-derived neurotrophic factor (GDNF) expression and PI3K/Akt pathway activation, which might ameliorate MPTP injection-induced progressive elevation of reactive oxygen species (ROS) formation in mice. In addition to the effect on ROS, puerarin ameliorated MPTP-reduced lysosome-associated membrane protein type 2A (Lamp 2A) expression. Taken together, our data demonstrate that puerarin attenuates MPTP-induced dopaminergic neuronal degeneration via modulating GDNF expression, PI3K/Akt pathway and GSH activation, which subsequently ameliorate MPTP-induced ROS formation and decrease of Lamp 2A expression. Copyright © 2013 John Wiley & Sons, Ltd.