Decursin Exerts Anti-cancer Activity in MDA-MB-231 Breast Cancer Cells Via Inhibition of the Pin1 Activity and Enhancement of the Pin1/p53 Association

Authors

  • Ji-Hyun Kim,

    1. College of Oriental Medicine, Kyung Hee University, Seoul, South Korea
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  • Ji Hoon Jung,

    1. College of Oriental Medicine, Kyung Hee University, Seoul, South Korea
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  • Sung-Hoon Kim,

    Corresponding author
    1. College of Oriental Medicine, Kyung Hee University, Seoul, South Korea
    • Correspondence to: Soo-Jin Jeong, Ph D. Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Yuseongdae-ro 1672, Yuseong-gu, Daejeon, 305-811, South Korea; Sung-Hoon Kim, O.M.D., Ph.D. College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 131-701, Republic of Korea.

      E-mail: sjijeong@kiom.re.kr, sungkim7@khu.ac.kr

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  • Soo-Jin Jeong

    Corresponding author
    1. Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, South Korea
    • Correspondence to: Soo-Jin Jeong, Ph D. Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Yuseongdae-ro 1672, Yuseong-gu, Daejeon, 305-811, South Korea; Sung-Hoon Kim, O.M.D., Ph.D. College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 131-701, Republic of Korea.

      E-mail: sjijeong@kiom.re.kr, sungkim7@khu.ac.kr

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Abstract

The peptidyl-prolyl cis/trans isomerase Pin1 is overexpressed in a wide variety of cancer cells and thus considered as an important target molecule for cancer therapy. This study demonstrates that decursin, a bioactive compound from Angelica gigas, exert the anti-cancer effect against breast cancer cells via regulation of Pin1 and its related signaling molecules. We observed that decursin induced G1 arrest with decrease in cyclin D1 level in Pin1-expressing breast cancer cells MDA-MB-231, but not Pin1-non-expressing breast cancer cells MDA-MB-157. In addition, decursin significantly reduced protein expression and enzymatic activity of Pin1 in MDA-MB-231 cells. Further, we found that decursin treatment enhanced the p53 expression level and failed to down-regulate Pin1 in the cells transfected with p53 siRNA, indicating the importance of p53 in the decursin-mediated Pin1 inhibition in MDA-MB-231 cells. Decursin stimulated association between Pin1 to p53. Moreover, decursin facilitated p53 transcription in MDA-MB-231 cells. Overall, our current study suggests the potential of decursin as an attractive cancer therapeutic agent for breast cancer by targeting Pin1 protein. Copyright © 2013 John Wiley & Sons, Ltd.

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