Hypopigmentary Effects of Ethyl P-Methoxycinnamate Isolated from Kaempferia galanga
Version of Record online: 23 APR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 28, Issue 2, pages 274–279, February 2014
How to Cite
Ko, H.-J., Kim, H. J., Kim, S. Y., Yun, H.-Y., Baek, K. J., Kwon, N. S., Wan Kyun Whang, Choi, H.-R., Park, K.-C. and Kim, D.-S. (2014), Hypopigmentary Effects of Ethyl P-Methoxycinnamate Isolated from Kaempferia galanga. Phytother. Res., 28: 274–279. doi: 10.1002/ptr.4995
- Issue online: 4 FEB 2014
- Version of Record online: 23 APR 2013
- Manuscript Accepted: 17 MAR 2013
- Manuscript Revised: 8 MAR 2013
- Manuscript Received: 28 JUN 2012
- Korea Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea. Grant Number: A100179
- Kaempferia galanga;
- ethyl p-methoxycinnamate;
- skin whitening
We isolated crystals from the chloroform fraction of an ethanol extract of Kaempferia galanga and identified it as ethyl p-methoxycinnamate through nuclear magnetic resonance analysis. In the present study, we found that ethyl p-methoxycinnamate significantly decreased melanin synthesis in B16F10 murine melanoma cells stimulated with α-melanocyte stimulating hormone (α-MSH). In a cell-free system, however, ethyl p-methoxycinnamate did not directly inhibit tyrosinase, the rate-limiting enzyme of melanogenesis. Instead, it inhibited tyrosinase activity in B16F10 cells in a dose-dependent manner. Furthermore, Western blot analysis showed that ethyl p-methoxycinnamate decreased microphthalmia-associated transcription factor and tyrosinase levels in α-MSH-stimulated B16F10 cells. These results indicate that the pigment-inhibitory effect of ethyl p-methoxycinnamate results from downregulation of tyrosinase. Ethyl p-methoxycinnamate isolated from K. galanga could be developed as a skin whitening agent to treat hyperpigmentary disorders. Copyright © 2013 John Wiley & Sons, Ltd.