Hypolipidemic Activity of Okra is Mediated Through Inhibition of Lipogenesis and Upregulation of Cholesterol Degradation
Article first published online: 22 APR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 28, Issue 2, pages 268–273, February 2014
How to Cite
Wang, H., Chen, G., Ren, D. and Yang, S.-T. (2014), Hypolipidemic Activity of Okra is Mediated Through Inhibition of Lipogenesis and Upregulation of Cholesterol Degradation. Phytother. Res., 28: 268–273. doi: 10.1002/ptr.4998
- Issue published online: 4 FEB 2014
- Article first published online: 22 APR 2013
- Manuscript Accepted: 21 MAR 2013
- Manuscript Revised: 26 FEB 2013
- Manuscript Received: 15 OCT 2012
- National Natural Science Foundation of China and Fundamental Research Funds for the Central Universities. Grant Numbers: 30800609, 31270085
- hypolipidemic activity;
Little is known about the hypolipidemic activity of okra; therefore, we investigated the hypolipidemic activity of okra and its interaction with gene expression of several key components involved in lipid homeostasis. Male C57BL/6 mice were randomly divided into three groups and fed with hyperlipidemic diet or two hyperlipidemic diets supplemented with 1% or 2% okra powder for eight weeks. Results demonstrated that okra dose-dependently decreased serum and hepatic total cholesterol and triglyceride, and enhanced fecal excretion of bile acids. Gene expression analysis revealed that okra upregulated cholesterol 7α-hydroxylase (CYP7A1) expression, downregulated expression of sterol regulatory element-binding protein 1c (SREBP1c) and fatty acid synthase (FAS), with no effect on sterol regulatory element-binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), low-density lipoprotein receptor (LDLR) and carnitine palmitoyltransferase-1A (CPT1A). It was suggested that hypolipidemic activity of okra was mediated most likely by upregulation of cholesterol degradation through CYP7A1 and by inhibition of lipogenesis through SREBP1c and FAS. Okra raw and fractionated polysaccharide showed strong bile acid binding capacity in vitro, which may contribute to the hypolipidemic activity observed. In conclusion, okra has potential application in the management of hyperlipidemia and its associated metabolic disorders. Copyright © 2013 John Wiley & Sons, Ltd.