Pronounced Effect of DNA Hybridization on Click Reaction Efficiency

Authors

  • Christian T. Wirges,

    1. Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstr. 5 – 13, Haus F, 81377 Munich, Germany, Tel.: 0049-89-2180-77755, Fax: 0049-89-2180-77756
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  • Philipp M. E. Gramlich,

    1. Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstr. 5 – 13, Haus F, 81377 Munich, Germany, Tel.: 0049-89-2180-77755, Fax: 0049-89-2180-77756
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  • Katrin Gutsmiedl,

    1. Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstr. 5 – 13, Haus F, 81377 Munich, Germany, Tel.: 0049-89-2180-77755, Fax: 0049-89-2180-77756
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  • Johannes Gierlich,

    1. Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstr. 5 – 13, Haus F, 81377 Munich, Germany, Tel.: 0049-89-2180-77755, Fax: 0049-89-2180-77756
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  • Glenn A. Burley,

    1. Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstr. 5 – 13, Haus F, 81377 Munich, Germany, Tel.: 0049-89-2180-77755, Fax: 0049-89-2180-77756
    2. Department of Chemistry, University of Leicester, Leicester, LE1 7RH, UK
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  • Thomas Carell

    1. Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstr. 5 – 13, Haus F, 81377 Munich, Germany, Tel.: 0049-89-2180-77755, Fax: 0049-89-2180-77756
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Abstract

We report about the efficiency of the click reaction directly on PCR products containing alkyne-modified uridine nucleosides. Galactose azide was clicked onto single-stranded and double-stranded 300mer DNA in order to investigate the efficiency of sugar labeling of DNA under various conditions. Single-stranded alkyne-modified DNA was prepared by selective lambda exonuclease digestion of 5′-phosphate-labeled double strands. A comparison of the click reaction efficiency on modified single and double stranded 300mers under identical conditions shows a pronounced effect of the strandedness on the yield of the click reaction.

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