Fast liquid chromatography coupled to electrospray tandem mass spectrometry peptide sequencing for cross-species protein identification
Article first published online: 29 AUG 2003
Copyright © 2003 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 17, Issue 19, pages 2188–2194, 15 October 2003
How to Cite
Witters, E., Laukens, K., Deckers, P., Dongen, W. V., Esmans, E. and Onckelen, H. V. (2003), Fast liquid chromatography coupled to electrospray tandem mass spectrometry peptide sequencing for cross-species protein identification. Rapid Commun. Mass Spectrom., 17: 2188–2194. doi: 10.1002/rcm.1173
- Issue published online: 29 AUG 2003
- Article first published online: 29 AUG 2003
- Manuscript Revised: 1 AUG 2003
- Manuscript Accepted: 1 AUG 2003
- Manuscript Received: 17 MAR 2003
- Interuniversity Attraction Poles Programme—Belgian State—Federal Office for Scientific Technical and Cultural Affairs
Using a parallel microcolumn switching liquid chromatography set-up coupled to a quadrupole time-of-flight mass spectrometer, a rapid liquid chromatography/mass spectrometric (LC/MS) protein identification method is presented. Without prior sample clean-up up to 300 protein digest samples a day can be processed. Using data-directed acquisition, up to 10 fragmentation analyses for each protein sample can be acquired in the same chromatographic run that can be used for database searching. Using internal peptide sequence information, protein databases and the various nucleic acid databases can both be queried for cross-species identification of the protein sample. The method was evaluated and put into force to generate data for a tobacco cell culture protein database. Copyright © 2003 John Wiley & Sons, Ltd.