Research Article

A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts

  1. Claude R. Mallet*,
  2. Ziling Lu,
  3. Jeff R. Mazzeo

Article first published online: 4 DEC 2003

DOI: 10.1002/rcm.1276

Issue

Rapid Communications in Mass Spectrometry

Rapid Communications in Mass Spectrometry

Volume 18, Issue 1, pages 49–58, 15 January 2004

Additional Information

How to Cite

Mallet, C. R., Lu, Z. and Mazzeo, J. R. (2004), A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts. Rapid Commun. Mass Spectrom., 18: 49–58. doi: 10.1002/rcm.1276

Author Information

  1. Waters Corporation, 34 Maple St., Milford, MA 01757-3696, USA

*Waters Corporation, 34 Maple St., Milford, MA 01757-3696, USA.

Publication History

  1. Issue published online: 4 DEC 2003
  2. Article first published online: 4 DEC 2003
  3. Manuscript Accepted: 22 OCT 2003
  4. Manuscript Revised: 20 OCT 2003
  5. Manuscript Received: 25 SEP 2003

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Abstract

Since the wide adoption of liquid chromatography/tandem mass spectrometry (LC/MS/MS), the ion suppression/enhancement phenomenon is the latest barrier to high-throughput analysis. This consequence of a nonoptimized analytical method can lead to adverse effects during quantitation (i.e. poor accuracy and precision). Previous papers have reported that ion suppression is a direct result of endogenous material present in biological samples. However, in the case of a solid-phase liquid chromatography/tandem mass spectrometry (SPE/LC/MS/MS) system, the measured result is the combination of several operating conditions and parameters. Little has been done to effectively monitor and/or choose optimized conditions for the complete sequence of extraction, clean up, separation and analysis. This paper describes a simple setup for quantification of ion suppression/enhancement. Several mobile phase additives, ion-pairing agents and SPE extracts were measured and compared against a standard reference. The results demonstrated that a clean up of plasma extracts based on ion exchange leads to minimal ion suppression/enhancement for the compounds that were investigated. Copyright © 2003 John Wiley & Sons, Ltd.

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