Negative ion mass spectrometry of silylated cytokinins

Authors

  • Charles H. Hocart,

    1. University of Bern, Institute of Organic Chemistry, Freiestrasse 3, CH-3012 Bern, Switzerland
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  • Urs P. Schlunegger

    Corresponding author
    1. University of Bern, Institute of Organic Chemistry, Freiestrasse 3, CH-3012 Bern, Switzerland
    • University of Bern, Institute of Organic Chemistry, Freiestrasse 3, CH-3012 Bern, Switzerland
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Abstract

The negative ion mass spectra or trimethylsilylzeatin riboside and t-butyldimethylsilylzeatin, generated using either ammonia or 10% CO2/Ar as reagent gas, are described. Optimal yields of [M-H] ions were obtained with a source pressure of 5 Pa in the electron ionization source of a ZAB-2F mass spectrometer, compared with 15 Pa to generate [M+H]+ in the chemical ionization mode. At 15 Pa no negative ions could be observed.

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