Matrix-assisted laser desorption of large biomolecules with a TEA-CO2-laser



Matrix-assisted desorption/ionization of large biomolecules with a TEA-CO2 laser with a warelength of 10.6 μm is demonstrated. TEA-CO2 LDI mass spectra are almost identical to those obtained with the Er-YAG (2.94 μm) infra-red (IR) laser and very similar to UV-LDI spectra. Most of the matrices usable for Er-YAG IR-LDI have been tested at 10.6 μm with success, and the main features of IR-LDI e.g., the tendency towards the formation of higher chargers is shown.