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Analysis of bovine β-casein tryptic digest by continuous-flow fast-atom bombardment mass spectrometry

Authors

  • D. S. Jones,

    Corresponding author
    1. Kratos Analytical, Barton Dock Road, Urmston, Manchester, M31 2LD, UK
    • Kratos Analytical, Barton Dock Road, Urmston, Manchester, M31 2LD, UK
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  • W. Heerma,

    1. Kratos Analytical, Barton Dock Road, Urmston, Manchester, M31 2LD, UK
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    • Laboratory of Analytical Chemistry, University of Utrecht, Croesestraat 77A, 3522 AD Utrecht, The Netherlands.

  • P. D. van Wassenaar,

    1. Kratos Analytical, Barton Dock Road, Urmston, Manchester, M31 2LD, UK
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    • Unilever Research Laboratory, PO Box 114, 3130 AC Vlaardingen, The Netherlands.

  • J. Haverkamp

    1. Kratos Analytical, Barton Dock Road, Urmston, Manchester, M31 2LD, UK
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    • Laboratory of Analytical Chemistry, University of Utrecht, Croesestraat 77A, 3522 AD Utrecht, The Netherlands.


Abstract

Liquid chromatography/fast-atom bombardment mass spectrometry was used to partially confirm the amino-acid sequence of the protein, β-casein. The study demonstrates that the technique is capable of the rapid and accurate identification of peptide fragments from tryptic digests and that chromatograrphic integrity is maintained during the analysis. The power of the technique derives from the ability to determine both the retention time and the molecular weight of the eluting components. Each of the components yields a prominent pseudo-molecular ion (MH+), the majority exhibiting sufficient fragmentation to confirm their structure unambiguously.

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