Liquid chromatography/fast-atom bombardment mass spectrometry was used to partially confirm the amino-acid sequence of the protein, β-casein. The study demonstrates that the technique is capable of the rapid and accurate identification of peptide fragments from tryptic digests and that chromatograrphic integrity is maintained during the analysis. The power of the technique derives from the ability to determine both the retention time and the molecular weight of the eluting components. Each of the components yields a prominent pseudo-molecular ion (MH+), the majority exhibiting sufficient fragmentation to confirm their structure unambiguously.
If you can't find a tool you're looking for, please click the link at the top of the page to "Go to old article view". Alternatively, view our Knowledge Base articles for additional help. Your feedback is important to us, so please let us know if you have comments or ideas for improvement.