Peptide sequencing by matrix-assisted laser-desorption mass spectrometry
Article first published online: 8 APR 2005
Copyright © 1992 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 6, Issue 2, pages 105–108, February 1992
How to Cite
Spengler, B., Kirsch, D., Kaufmann, R. and Jaeger, E. (1992), Peptide sequencing by matrix-assisted laser-desorption mass spectrometry. Rapid Commun. Mass Spectrom., 6: 105–108. doi: 10.1002/rcm.1290060207
- Issue published online: 8 APR 2005
- Article first published online: 8 APR 2005
- Manuscript Received: 4 DEC 1991
- Manuscript Accepted: 4 DEC 1991
A novel method of peptide sequencing by mass spectrometry is described. Metastable decay of laser-desorbedions, taking place in the first field-free drift region of a reflectron time-of-flight mass spectrometer, has been monitored to get structural information from larger peptides. Fragment ions from metastable decay are mass analysed by adjusting the potentiaals of the ion reflectron according to the kinetic energies of the ions. The features of the technique and its significance for future applications are outlined.