Get access

Electrospray ionization mass spectrometry coupled with size-exclusion chromatography

Authors

  • Laszlo Prokai,

    Corresponding author
    1. University of Florida, Center for Drug Discovery, Gainesville, FL 32610-0497, USA
    • University of Florida, Center for Drug Discovery, Gainesville, FL 32610-0497, USA
    Search for more papers by this author
  • William J. Simonsick Jr.

    1. E.I. duPont de Nemours & Co., Marshall R&D Laboratory, Philadelphia, Pennsylvania, USA
    Search for more papers by this author

Abstract

The coupling of electrospray ionization mass spectrometry with gel permeation chromatography (GPC) has been used for the separation of a commercial polydisperse mixture of octylphenoxypoly(ethoxy)ethanol. Stable electrospray conditions are obtained upon using, as GPC solvent, tetrahydrofuran containing 10−5−10−4M sodium salt which also provides for the formation of intact molecular ions through cationization. Selected-ion chromatograms of sodiated species allow for the monitoring of the elution profiles of individual oligomers, and can be used for the calibration of the GPC.

Get access to the full text of this article

Ancillary