Multiply pulsed collision gas for ion axialization in fourier-transform ion cyclotron resonance mass spectrometry

Authors

  • Shengheng Guan,

    1. Department of Chemistry, 120 West 18th Avenue. The Ohio State University, Columbus OH 43210, USA
    Search for more papers by this author
  • Alan G. Marshall

    Corresponding author
    1. Department of Chemistry, 120 West 18th Avenue. The Ohio State University, Columbus OH 43210, USA
    Current affiliation:
    1. National High Field Laboratory, Florida State University. 1800 East Paul Dirac Drive, PO Box 4005, Tallahassee, FL 32306-4005, USA
    • Department of Chemistry, 120 West 18th Avenue. The Ohio State University, Columbus OH 43210, USA
    Search for more papers by this author
    • Member of the Department of Biochemistry.


Abstract

We present a new multiply pulsed collision-gas technique forion axialization for Fourier-transform ion cyclotron resonace (FTICR) high-resolution mass analysis. Ions are axialized in the source trap of a dural-trap FTICR spectrometer y aimuthal quadrupolar irradiation for several tens of seconds at a steady-state pressure maintained y repeated pulses of coolision gas (argon), folowed by removal of the collision gas and detection at a much lower pressure after transfer to the analyzer trap. Magnitufe-mode FTICR mass resolving power, m/Δm≈1770000, for [M + K]+ ions from laser-desorbed/ionized leucne enkephalin at m/z 594 is demonstrated. This resolving power is the highest yet reported for a peptide with any type of laser desorption/ionization mass analyzer.

Ancillary