Presented at the 11th Annual Meeting of the French Mass Spectrometry Society, held at Rouen, 20–22 September 1994.
Electrospray mass spectrometry for the characterization of the purity of natural and modified oligodeoxynucleotides†
Article first published online: 8 APR 2005
Copyright © 1995 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 9, Issue 1, pages 1–4, 1995
How to Cite
Deroussent, A., Le Caer, J.-P., Rossier, J. and Gouyette, A. (1995), Electrospray mass spectrometry for the characterization of the purity of natural and modified oligodeoxynucleotides. Rapid Commun. Mass Spectrom., 9: 1–4. doi: 10.1002/rcm.1290090102
- Issue published online: 8 APR 2005
- Article first published online: 8 APR 2005
- Manuscript Accepted: 3 NOV 1994
- Manuscript Received: 30 OCT 1994
Electrospray ionization mass spectrometry is an accurate and sensitive analytical method to characterize the purity of oligodeoxynucleotides being tested for pharmacological studies. We report the preparation procedure (‘desalting’) of natural and modified oligodeoxynucleotides (ODNs) and their analysis by negative-ion electrospray mass spectrometry. We evaluate the sensitivity and the accuracy of the method for two antisense ODN sequences. Mass analysis of the 25-mer phosphorothioate can be performed to within 0.001% accuracy (standard error of 0.05 Da) for a sample concentration of 12 pmol/μL. In addition, the adduct ion and the failure sequence can be identified to characterize the antisense ODN.