Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of DNA–Pt(II) complexes

Authors

  • J. Guittard,

    Corresponding author
    1. Université Pierre et Marie Curie, Laboratoire de Chimie Organique Structurale, CNRS ERS 73, 4 Place Jussieu, F-75252 Paris Cedex 05, France
    • Université Pierre et Marie Curie, Laboratoire de Chimie Organique Structurale, CNRS ERS 73, 4 Place Jussieu, F-75252 Paris Cedex 05, France
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  • C. Pacifico,

    1. Institut Curie, Laboratoire de Physique et Chimie Biomoléculaires, CNRS URA 198, 11 rue Pierre et Marie Curie, F-75251 Paris Cedex 05, France
    2. Université Paris 5, Laboratoire de Chimie et Biochimic Pharmacologiques et Toxycologiques, CNRS URA 400, 45 rue des Saints-Pères, F-75270 Paris Cedex 06, France
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  • J. C. Blais,

    1. Université Pierre et Marie Curie, Laboratoire de Chimie Organique Structurale, CNRS ERS 73, 4 Place Jussieu, F-75252 Paris Cedex 05, France
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  • G. Bolbach,

    1. Université Pierre et Marie Curie, Laboratoire de Chimie Organique Structurale, CNRS ERS 73, 4 Place Jussieu, F-75252 Paris Cedex 05, France
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  • J. C. Chottard,

    1. Université Paris 5, Laboratoire de Chimie et Biochimic Pharmacologiques et Toxycologiques, CNRS URA 400, 45 rue des Saints-Pères, F-75270 Paris Cedex 06, France
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  • A. Spassky

    1. Institut Curie, Laboratoire de Physique et Chimie Biomoléculaires, CNRS URA 198, 11 rue Pierre et Marie Curie, F-75251 Paris Cedex 05, France
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  • Presented at the 11th Annual Meeting of the French Mass Spectrometry Society, held at Rouen, 20–22 September 1994.

Abstract

Matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry has been used to characterize the reaction products of the 18-mer deoxyribonucleotide d(AACGGTTAACCGTTAATT) with [Pt(NH3)3(H2O)]2+ and cis-[Pt(NH3)2(H2O)2]2+. Characteristic peaks corresponding to different monofunctional adducts {18-mer+n[Pt(NH3)3]} (n =1, 2, 3 and 4) have been observed with the triamino–monoaqua complex. With the diamino-diaqua cis-Pt complex, formation of a chelate {18-mer+[Pt(NH3)2]} involving two adjacent guanines has been demonstrated. A good correlation between MALDI and polyacrylamide gel electrophoresis results is observed.

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