Direct analysis of selected N-acyl-L-homoserine lactones by gas chromatography/mass spectrometry
Article first published online: 14 MAY 2004
Copyright © 2004 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 18, Issue 12, pages 1341–1344, 30 June 2004
How to Cite
Cataldi, T. R. I., Bianco, G., Frommberger, M. and Schmitt-Kopplin, Ph. (2004), Direct analysis of selected N-acyl-L-homoserine lactones by gas chromatography/mass spectrometry. Rapid Commun. Mass Spectrom., 18: 1341–1344. doi: 10.1002/rcm.1480
- Issue published online: 14 MAY 2004
- Article first published online: 14 MAY 2004
- Manuscript Revised: 6 APR 2004
- Manuscript Accepted: 6 APR 2004
- Manuscript Received: 25 FEB 2004
A rapid, simple and selective method involving direct separation by gas chromatography (GC) with electron ionization mass spectrometry (EI-MS) was employed to determine some N-acylhomoserine lactones (AHLs). Using GC/EI-MS, simultaneous separation and characterization of AHLs were possible without prior derivatization. Informative fragmentation patterns were obtained to identify the structures of N-acyl chains of AHLs. Electron ionization resulted in a common fragmentation pattern with the most abundant ion at m/z 143 and other minor peaks at m/z 71, 57, and 43. The presence of AHLs in extracts of Burkholderia cepacia strains was achieved in selected ion monitoring mode by using the prominent fragment at m/z 143. Copyright © 2004 John Wiley & Sons, Ltd.