Capillary electrophoresis off-line matrix-assisted laser desorption/ionisation mass spectrometry of intact and digested proteins using cationic-coated capillaries
Article first published online: 4 NOV 2004
Copyright © 2004 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 18, Issue 23, pages 2946–2952, 15 December 2004
How to Cite
Zuberovic, A., Ullsten, S., Hellman, U., Markides, K. E. and Bergquist, J. (2004), Capillary electrophoresis off-line matrix-assisted laser desorption/ionisation mass spectrometry of intact and digested proteins using cationic-coated capillaries. Rapid Commun. Mass Spectrom., 18: 2946–2952. doi: 10.1002/rcm.1712
- Issue published online: 4 NOV 2004
- Article first published online: 4 NOV 2004
- Manuscript Accepted: 8 OCT 2004
- Manuscript Revised: 28 SEP 2004
- Manuscript Received: 23 JUN 2004
- Swedish Strategic Foundation. Grant Number: K-5104-706/2001
- Swedish Research Council. Grant Number: 621-2002-5261
Capillary electrophoresis (CE) was coupled off-line with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) for the analysis of proteins and peptides. CE fractions were collected directly on a matrix-coated MALDI target, using a sheath-flow interface. Protein adsorption during CE separations was prevented by coating the capillaries with the physically adsorbed, cationic polymer PolyE-323. The CE/MALDI-MS system was used for the analysis of model proteins and peptides at physiological pH as well as analysis of proteins in tear fluid. Moreover, tryptic on-target digestion of the collected protein fractions, with subsequent MALDI-MS and MS/MS peptide analysis, was demonstrated. Copyright © 2004 John Wiley & Sons, Ltd.