Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis
Article first published online: 9 MAY 2005
Copyright © 2005 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 19, Issue 11, pages 1529–1535, 15 June 2005
How to Cite
Strano-Rossi, S., Molaioni, F., Rossi, F. and Botrè, F. (2005), Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis. Rapid Commun. Mass Spectrom., 19: 1529–1535. doi: 10.1002/rcm.1942
- Issue published online: 11 MAY 2005
- Article first published online: 9 MAY 2005
- Manuscript Revised: 24 MAR 2005
- Manuscript Accepted: 24 MAR 2005
- Manuscript Received: 31 JAN 2005
- Italian Department of Health—National Antidoping Commission
This paper describes a rapid gas chromatographic/mass spectrometric (GC/MS) screening method for the detection of drugs of abuse and/or their metabolites in urine. Synthetic stimulants, opiates, cocaine metabolites, cannabinoids—and specifically the acid metabolite of tetrahydrocannabinol (THC-COOH)—can be simultaneously extracted by a single liquid/liquid separation step, at alkaline pH, and assayed as trimethylsilyl derivatives by GC/MS in SIM (selected ion monitoring) mode. All the analytes show a good linearity (R2 > 0.99 for most of the considered substances) in the range 25–1000 ng/mL, with a good reproducibility of both the retention times (CV% <0.7) and the relative abundances of the characteristic diagnostic ions (CV% <13). The limit of detection (LOD) of the method is 25 ng/mL of target compound in human urine for most of the substances investigated, 3 ng/mL for THC-COOH, and 10 ng/mL for norbuprenorphine. Validation of the method allows its application to different fields of forensic analytical toxicology, including antidoping analysis. Copyright © 2005 John Wiley & Sons, Ltd.