Direct analysis of laser capture microdissected endometrial carcinoma and epithelium by matrix-assisted laser desorption/ionization mass spectrometry

Authors

  • Jingzhong Guo,

    1. Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
    Search for more papers by this author
  • Terence J. Colgan,

    1. Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    2. Department of Laboratory Medicine and Pathobiology, University of Toronto, 100 College Street, Toronto, Ontario, Canada M5G 1L5
    Search for more papers by this author
  • Leroi V. DeSouza,

    1. Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
    Search for more papers by this author
  • Mary Joe Rodrigues,

    1. Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Search for more papers by this author
  • Alexander D. Romaschin,

    1. Department of Laboratory Medicine and Pathobiology, University of Toronto, 100 College Street, Toronto, Ontario, Canada M5G 1L5
    2. Biochemistry, Toronto General Hospital, 200 Elizabeth Street, Toronto, Ontario, Canada M5G 2C4
    Search for more papers by this author
  • K. W. Michael Siu

    Corresponding author
    1. Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
    • Department of Chemistry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
    Search for more papers by this author

Abstract

Direct analysis of laser capture microdissected malignant and normal endometrial epithelium using matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (MS) was able to detect a number of proteins that are overexpressed in malignant epithelial cells. A total of 16 physiologic and malignant endometrial samples were laser capture microdissected, including four proliferative and four secretory endometria, and eight endometrioid adenocarcinomas. Two of these proteins, at 10 834 and 10 843 Da, likely correspond to calgranulin A and chaperonin 10, two proteins that had previously been identified in endometrioid adenocarcinoma in whole tissue homogenate by MS analysis. Direct analysis by MALDI-MS not only confirms that these proteins are overexpressed in endometrial carcinoma, but also localizes them to the epithelial cells, the expected cancer site. Copyright © 2005 John Wiley & Sons, Ltd.

Ancillary