Specific photodissociation of peptides with multi-stage mass spectrometry

Authors

  • Thibault Tabarin,

    1. Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France
    Search for more papers by this author
  • Rodolphe Antoine,

    Corresponding author
    1. Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France
    • Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
    Search for more papers by this author
  • Michel Broyer,

    1. Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France
    Search for more papers by this author
  • Philippe Dugourd

    1. Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France
    Search for more papers by this author

Abstract

We report collision-induced dissociation (CID) and laser-induced dissociation (LID) performed at different wavelengths between 220 and 280 nm of the peptides leucine-enkephalin (protonated) and gramicidin A (sodiated). Hydrogen-atom losses and side-chain cleavages were observed in LID experiments. These losses depend on the laser wavelength and lead to the formation of radical ions. The fragmentations of these radicals, which are not observed in CID experiments, were investigated in multi-stage mass spectrometry experiments. Copyright © 2005 John Wiley & Sons, Ltd.

Ancillary