Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids
Article first published online: 18 NOV 2005
Copyright © 2005 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 19, Issue 24, pages 3701–3704, 30 December 2005
How to Cite
Shiea, J., Huang, M.-Z., HSu, H.-J., Lee, C.-Y., Yuan, C.-H., Beech, I. and Sunner, J. (2005), Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids. Rapid Commun. Mass Spectrom., 19: 3701–3704. doi: 10.1002/rcm.2243
- Issue published online: 18 NOV 2005
- Article first published online: 18 NOV 2005
- Manuscript Revised: 16 OCT 2005
- Manuscript Accepted: 16 OCT 2005
- Manuscript Received: 1 SEP 2005
A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds. Copyright © 2005 John Wiley & Sons, Ltd.