Research Article

Desorption electrospray ionization mass spectrometry for the analysis of pharmaceuticals and metabolites

  1. Tiina J. Kauppila1,2,
  2. Justin M. Wiseman1,
  3. Raimo A. Ketola2,
  4. Tapio Kotiaho3,
  5. R. Graham Cooks1,*,
  6. Risto Kostiainen2,*

Article first published online: 28 DEC 2005

DOI: 10.1002/rcm.2304

Issue

Rapid Communications in Mass Spectrometry

Rapid Communications in Mass Spectrometry

Volume 20, Issue 3, pages 387–392, 15 February 2006

Additional Information

How to Cite

Kauppila, T. J., Wiseman, J. M., Ketola, R. A., Kotiaho, T., Cooks, R. G. and Kostiainen, R. (2006), Desorption electrospray ionization mass spectrometry for the analysis of pharmaceuticals and metabolites. Rapid Communications in Mass Spectrometry, 20: 387–392. doi: 10.1002/rcm.2304

Author Information

  1. 1

    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA

  2. 2

    Division of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Helsinki, 00014 Helsinki, Finland

  3. 3

    Department of Chemistry, University of Helsinki, 00014 Helsinki, Finland

*Department of Chemistry, 560 Oval Drive, Purdue University, West Lafayette, IN 47907-2084, USA.

Publication History

  1. Issue published online: 28 DEC 2005
  2. Article first published online: 28 DEC 2005
  3. Manuscript Revised: 17 NOV 2005
  4. Manuscript Accepted: 17 NOV 2005
  5. Manuscript Received: 26 AUG 2005

Funded by

  • Academy of Finland and KCL
  • NIH/NIGMS. Grant Number: 5R01 GM58008-07

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Abstract

The performance of desorption electrospray ionization (DESI) in the analysis of a group of pharmaceuticals and their glucuronic acid conjugates is reported. The suitability of different sprayer solvents and different surfaces was examined. In the positive ion mode, water/methanol/trifluoroacetic acid performed best, whereas, in the negative ion mode, water/methanol/ammonium hydroxide was found to be the most suitable spray solvent. Of the surfaces investigated, polymethylmethacrylate (PMMA) was found to give the best performance in terms of sensitivity. Spray solution flow rate and the distance of the sprayer tip from the surface were also found to have significant effects on the signal intensity. Analytes with basic groups efficiently formed the corresponding protonated molecules in the positive ion mode, whereas acidic analytes, such as the glucuronic acid conjugates, formed intense signals due to the deprotonated molecules in the negative ion mode. Ionization of neutral compounds was less efficient and in many cases it was achieved through adduct formation with simple anions or cations. Copyright © 2005 John Wiley & Sons, Ltd.

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