Get access

A new hybrid electrospray Fourier transform mass spectrometer: design and performance characteristics

Authors

  • Peter B. O'Connor,

    Corresponding author
    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    2. Cardiovascular Proteomics Center, Boston University School of Medicine, 650 Albany St. X-116, Boston, MA 02118-2526, USA
    • Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA.
    Search for more papers by this author
  • Jason L. Pittman,

    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    Search for more papers by this author
  • Bruce A. Thomson,

    1. MDS Sciex, 71 Four Valley Drive, Concord, ON, L4K 4V8 Canada
    Search for more papers by this author
  • Bogdan A. Budnik,

    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    2. Cardiovascular Proteomics Center, Boston University School of Medicine, 650 Albany St. X-116, Boston, MA 02118-2526, USA
    3. Department of Pathology, Children's Hospital Boston, Harvard Medical School, 320 Longwood Av., E-1154, Boston, MA 02115, USA
    Search for more papers by this author
  • Jason C. Cournoyer,

    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    2. Department of Chemistry, Boston University, 590 Commonwealth Ave, Boston MA 02215, USA
    Search for more papers by this author
  • Judith Jebanathirajah,

    1. Department of Systems Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    Search for more papers by this author
  • Cheng Lin,

    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    Search for more papers by this author
  • Susanne Moyer,

    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    2. Agilent Technologies, 2850 Centerville Rd., Wilmington, DE 19808, USA
    Search for more papers by this author
  • Cheng Zhao

    1. Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA
    Search for more papers by this author

Abstract

A new hybrid electrospray quadrupole Fourier transform mass spectrometry (FTMS) instrument design is shown and characterized. This instrument involves coupling an electrospray source and mass-resolving quadrupole, ion accumulation, and collision cell linear ion trap system developed by MDS Sciex with a home-built ion guide and ion cyclotron resonance (ICR) cell. The iterative progression of this design is shown. The final design involves a set of hexapole ion guides to transfer the ions from the accumulation/collision trap through the magnetic field gradient and into the cell. These hexapole ion guides are separated by a thin gate valve and two conduction limits to maintain the required <10−9 mbar vacuum for FTICR. Low-attomole detection limits for a pure peptide are shown, 220 000 resolving power in broadband mode and 820 000 resolving power in narrow-band mode are demonstrated, and mass accuracy in the <2 ppm range is routinely available provided the signal is abundant, cleanly resolved, and internally calibrated. This instrument design provides high experimental flexibility, allowing Q2 CAD, SORI-CAD, IRMPD, and ECD experiments with selected ion accumulation as well as experiments such as nozzle skimmer dissociation. Initial top-down mass spectrometry experiments on a protein is shown using ECD. Copyright © 2005 John Wiley & Sons, Ltd.

Get access to the full text of this article

Ancillary