Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry
Article first published online: 14 FEB 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 20, Issue 6, pages 1001–1012, 30 March 2006
How to Cite
Jiang, H., Somogyi, Á., Jacobsen, N. E., Timmermann, B. N. and Gang, D. R. (2006), Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry. Rapid Commun. Mass Spectrom., 20: 1001–1012. doi: 10.1002/rcm.2401
- Issue published online: 14 FEB 2006
- Article first published online: 14 FEB 2006
- Manuscript Revised: 18 JAN 2006
- Manuscript Accepted: 18 JAN 2006
- Manuscript Received: 19 APR 2005
- National Science Foundation Plant Genome Program. Grant Number: DBI-0227618
- National Institutes of Health NCCAM/ODS. Grant Numbers: 5 P50 AT 000474-04, 3 P50 AT 000474-03 S1
The curcuminoids are a group of diarylheptanoid molecules that possess important pharmacological activities, particularly acting as anti-inflammatory agents. The main purpose of this study was to investigate the fragmentation behavior of the three major curcuminoids in ion trap liquid chromatography/tandem mass spectrometry (LC/MS/MS). Both positive and negative mode electrospray ionization in tandem and multidimensional MSn experiments in quadrupole ion trap instruments and high-resolution and accurate mass MS and sustained off-resonance irradiation (SORI) MS/MS experiments in a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer were used to elucidate the main fragmentation channels of these compounds. These experiments yielded essentially the same fragmentation results in both ion trap and ICR instruments for all three curcuminoids and for their phenolic monoacetates. Major and diagnostic fragment ions were identified and their origins are proposed. Copyright © 2006 John Wiley & Sons, Ltd.