Analyses of Stemona alkaloids in Stemona tuberosa by liquid chromatography/tandem mass spectrometry

Authors

  • Yan Zhou,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
    2. Chengdu Institute of Biology, The Chinese Academy of Sciences, Chengdu 610041, Sichuan Province, P.R. China
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  • Ren-Wang Jiang,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
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  • Po-Ming Hon,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
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  • Yan-Tong Xu,

    1. Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
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  • Yiu-Man Chan,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
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  • Tak-Wah Dominic Chan,

    1. Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
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  • Hong-Xi Xu,

    1. Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong Science Park, Shatin, New Territories, Hong Kong, P.R. China
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  • Li-Sheng Ding,

    1. Chengdu Institute of Biology, The Chinese Academy of Sciences, Chengdu 610041, Sichuan Province, P.R. China
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  • Paul Pui-Hay But,

    Corresponding author
    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
    2. Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
    • Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China.
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  • Pang-Chui Shaw

    Corresponding author
    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
    2. Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China
    • Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P.R. China.
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Abstract

Alkaloid profiles in Stemona tuberosa were found to be highly variable. Six Stemona alkaloids isolated from the plant were subjected to on-line high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) and tandem mass spectrometry (MS/MS) analyses. Their fragmentation patterns and products were useful for their characterization. The LC/MS fingerprints of these alkaloids, though variable among samples, could provide an overall characterization of the authenticity and quality of this species and help to differentiate it from S. japonica and S. sessilifolia, as all three species are recognized as genuine sources of the herb Radix Stemonae in the Pharmacopoeia of the People's Republic of China. Copyright © 2006 John Wiley & Sons, Ltd.

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