Simultaneous analysis of prostanoids using liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Authors

  • James Kapron,

    Corresponding author
    1. Ionalytics, M50-IPF, 1200 Montreal Rd, Ottawa, ON, K1A 0R6 Canada
    Current affiliation:
    1. Thermo Electron Corporation, M50-IPF, 1200 Montreal Rd., Ottawa, ON, K1A 0R6, Canada.
    • Thermo Electron Corporation, M50-IPF, 1200 Montreal Rd, Ottawa, ON, K1A 0R6, Canada.
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  • Jin Wu,

    1. Merck Frosst Centre for Therapeutic Research, 16711 Trans Canada Highway, Kirkland, Quebec, H9H 3L1 Canada
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  • Timo Mauriala,

    1. Merck Frosst Centre for Therapeutic Research, 16711 Trans Canada Highway, Kirkland, Quebec, H9H 3L1 Canada
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  • Patsy Clark,

    1. Merck Frosst Centre for Therapeutic Research, 16711 Trans Canada Highway, Kirkland, Quebec, H9H 3L1 Canada
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  • Randy W. Purves,

    1. Ionalytics, M50-IPF, 1200 Montreal Rd, Ottawa, ON, K1A 0R6 Canada
    Current affiliation:
    1. Thermo Electron Corporation, M50-IPF, 1200 Montreal Rd., Ottawa, ON, K1A 0R6, Canada.
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  • Kevin P. Bateman

    Corresponding author
    1. Merck Frosst Centre for Therapeutic Research, 16711 Trans Canada Highway, Kirkland, Quebec, H9H 3L1 Canada
    • Merck Frosst Canada Inc., P.O. Box 1005, Pointe Claire-Dorval, Quebec, H9R 4P8, Canada.
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  • Dedicated to Dr. Robert Boyd on the occasion of his retirement as Editor-in-Chief of RCM.

Abstract

A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E2, PGD2, PGF, 6-keto-PGF, and thromboxane (TX) B2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE2 and PGD2, and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate. Copyright © 2006 John Wiley & Sons, Ltd.

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